Human Serine, Threonine-protein Kinase 4, STK4 ELISA Kit

SKU: E4154hu

Size:96 Tests-1 Kit

Background:Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2. Phosphorylates TNNI3 (cardiac Tn-I) and alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylates FOXO1 on 'Ser-212' and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner. Phosphorylates SIRT1 and inhibits SIRT1-mediated p53/TP53 deacetylation, thereby promoting p53/TP53 dependent transcription and apoptosis upon DNA damage. Acts as an inhibitor of PKB/AKT1. Phosphorylates AR on 'Ser-650' and suppresses its activity by intersecting with PKB/AKT1 signaling and antagonizing formation of AR-chromatin complexes.

Research_area:Others

Applications:ELISA

Target_protein:STK4

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.021ng/ml

Detection range:0.05-10ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Serine/Threonine-Protein Kinase 4

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q13043

MASS(Da):55,630

GeneID:6789

Synonyms:Mammalian STE20-like protein kinase 1;MST-1;MST1/C;MST1/N;Serine/threonine-protein kinase 4;Serine/threonine-protein kinase 4 18kDa subunit;Serine/threonine-protein kinase 4 37kDa subunit;Serine/threonine-protein kinase Krs-2;STE20-like kinase MST1;STK 4;STK4

Gene names:STK4

Standard solution:12.8ng/ml