Human Protein lin-28 homolog A (LIN28) ELISA Kit

SKU: AE35508HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: Q9H9Z2

Abbreviation: LIN28

Alternative Names: CSDD1; FLJ12457; LIN-28; LIN28A; ZCCHC1; RNA-binding protein LIN-28|lin-28 homolog|zinc finger; CCHC domain containing 1

Application: ELISA

Range: 62.5-4000 pg/mL

Sensitivity: 23.3 pg/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate LIN28 in samples. An antibody specific for LIN28 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLIN28 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LIN28 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIN28 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: LIN28 protein is stage-specifically expressed in embryonic muscle, neurons, and epithelia, as well as in embryonic carcinoma cells; however, it is suppressed in most adult tissues except for skeletal and cardiac muscle.LIN28 mRNA is a target of the micro RNAs miR-125b and miRNA-125a.When the miREs were deleted, miRNA control over LIN28 nearly vanished. Lin28 downregulation by miR-125 involved reductions in both translational efficiency and mRNA abundance.An important target of Lin28 is Igf2, a growth and differentiation factor for muscle tissue. Interaction of Lin28 with translation initiation complexes in skeletal myoblasts and in the embryonic carcinoma cell line P19 was confirmed by localization of Lin28 to the stress granules, temporary structures that contain stalled mRNA-protein translation complexes.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).