Human Neuroendocrine convertase 2 (PCSK2) ELISA Kit

SKU: AE28357HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: P16519

Abbreviation: PCSK2

Alternative Names: NEC2; PC2; SPC2; KEX2-like endoprotease 2|neuroendocrine convertase 2|prohormone convertase 2|subtilisin-like prohormone convertases

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.112 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate PCSK2 in samples. An antibody specific for PCSK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCSK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCSK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCSK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Proprotein convertase 2 is a serine protease and proprotein convertase PC2, like proprotein convertase 1 (PC1), is an enzyme responsible for the first step in the maturation of many neuroendocrine peptides from their precursors, such as the conversion of proinsulin to insulin intermediates. To generate the bioactive form of insulin (and many other peptides), a second step involving the removal of C-terminal basic residues is required; this step is mediated by carboxypeptidases E and/or D. PC2 plays only a minor role in the first step of insulin biosynthesis, but a greater role in the first step of glucagon biosynthesis compared to PC1. PC2 binds to the neuroendocrine protein named 7B2, and if this protein is not present, proPC2 cannot become enzymatically active.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).