Human Late cornified envelope protein 3B (LCE3B) ELISA Kit

SKU: AE35903HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: Q5TA77

Abbreviation: LCE3B

Alternative Names: LEP14; late envelope protein 14

Application: ELISA

Range: 25-1600 pg/mL

Sensitivity: 6.25 pg/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate LCE3B in samples. An antibody specific for LCE3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLCE3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LCE3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LCE3B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: By database analysis to identify human orthologs of mouse genes encoding late envelope proteins (LEPs), Marshall et al. (2001) identified LCE3B, which they called LEP14. RT-PCR detected strong LEP14 expression in human esophagus, but not in skin or heart.Using real-time PCR, Jackson et al. (2005) detected little to no LCE3B expression in human skin or internal epithelia. Jackson et al. (2005) determined that the LCE3B gene contains 2 exons. Exon 1 is noncoding.By genomic sequence analysis, Marshall et al. (2001) mapped the LCE3B gene within the LCE gene cluster on chromosome 1q21. Jackson et al. (2005) stated that the mouse Lce3b gene maps to a syntenic LCE gene cluster on chromosome 3F1.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).