Human KLK10(Kallikrein 10) ELISA Kit

SKU: ELK1110

Size:96T

Reactivity:Human

Alternative Names:NES1; PRSSL1; Kallikrein-Related Peptidase 10; Normal epithelial cell-specific 1; Protease serine-like 1

Assay Type:Competitive Inhibition

Sensitivity:48.8 pg/mL

Standard:10000 pg/mL

Detection range:156.25-10000 pg/mL

Sample type:Serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Enzyme & Kinase;Tumor immunity;Reproductive science;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK10 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:O43240