Human Keratin, type II cytoskeletal 8 (KRT8) ELISA Kit

SKU: AE36249HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: P05787

Abbreviation: KRT8

Alternative Names: CARD2; CK8; CYK8; K2C8; K8; KO; cytokeratin 8|keratin; type II cytoskeletal 8

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.057 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate KRT8 in samples. An antibody specific for KRT8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. The human and mouse CK8 share about 82% identity in the N-terminal head domain, 95% identity in the alpha helical rod domain, and 67.5% identity in the C-terminal tail.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).