Human Keratin, type II cytoskeletal 5 (KRT5) ELISA Kit

SKU: AE36288HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: P13647

Abbreviation: KRT5

Alternative Names: CK5; DDD; EBS2; K5; KRT5A; 58 kda cytokeratin|epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types|keratin 5 (epidermolysis bullosa simplex; Dowling-Meara/Kobner/Weber-Cockayne

Application: ELISA

Range: 15.6-1000 pg/mL

Sensitivity: 6.1 pg/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate KRT5 in samples. An antibody specific for KRT5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic (i.e., different types of) keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. The type II cytokeratin genes are clustered in a region of chromosome 12q12-q13. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Eckert and Rorke (1988) cloned full-length KRT5 from a cultured keratinocyte cDNA library. The deduced 590-amino acid protein contains a central alpha-helical core region of about 310 amino acids with a 7-amino acid repeat found in coiled-coil structures. The N and C termini are serine rich and contain gly-gly-gly-x motifs.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).