Human Hydroxy-Delta-5-Steroid Dehydrogenase, 3 Beta- And Steroid Delta-Isomerase 1,HSD3B1 ELISA KIT

SKU: E7353Hu

Size:96 Tests-1 Kit

Background:A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone (PubMed:1401999, PubMed:2139411). Expected to use NAD(+) as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable). {ECO:0000269|PubMed:1401999, ECO:0000269|PubMed:2139411, ECO:0000305|PubMed:1401999}.

Research_area:

Applications:ELISA

Target_protein:HSD3B1

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.28ng/ml

Detection range:0.5-32ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:3 beta-hydroxysteroid dehydrogenase;Delta 5-->4-isomerase type 1

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P14060

MASS(Da):42252

GeneID:3283

Synonyms:HSD3B1 3BH HSDB3A

Gene names:HSD3B1

Standard solution:64ng/ml