Human GM2A(GM2 Ganglioside Activator) Microsample ELISA Kit

SKU: ELK6148MS

Size:96T

Reactivity:Human

Alternative Names:SAP-3; Cerebroside Sulfate Activator Protein; Sphingolipid Activator Protein 3

Assay Type:Sandwich

Sensitivity:0.065 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:Serum, plasma, tissue homogenates and other biological fluids

Assay length:3.5h

Research Area:Immune molecule;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GM2A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GM2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GM2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GM2A in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P17900