Human Glucosylceramidase,GBA ELISA KIT

SKU: E5333Hu

Size:96 Tests-1 Kit

Background:Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose (PubMed:9201993, PubMed:24211208, PubMed:15916907). Thereby, plays a central role in the degradation of complex lipids and the turnover of cellular membranes (PubMed:27378698). Through the production of ceramides, participates in the PKC-activated salvage pathway of ceramide formation (PubMed:19279011). Also plays a role in cholesterol metabolism (PubMed:24211208, PubMed:26724485). May either catalyze the glucosylation of cholesterol, through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol (PubMed:24211208, PubMed:26724485). The short chain saturated C8:0-GlcCer and the mono-unsaturated C18:0-GlcCer being the most effective glucose donors for that transglucosylation reaction (PubMed:24211208). Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide (PubMed:26724485). Finally, may also hydrolyze cholesteryl-beta-D-glucoside to produce D-glucose and cholesterol (PubMed:24211208, PubMed:26724485). {ECO:0000269|PubMed:15916907, ECO:0000269|PubMed:19279011, ECO:0000269|PubMed:24211208, ECO:0000269|PubMed:26724485, ECO:0000269|PubMed:27378698, ECO:0000269|PubMed:9201993}.

Research_area:

Applications:ELISA

Target_protein:GBA

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.049ng/ml

Detection range:0.1-40ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Glucosylceramidase

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P04062

MASS(Da):59716

GeneID:2629

Synonyms:GBA GC GLUC

Gene names:GBA

Standard solution:48ng/ml