Human GIF(Gastric Intrinsic Factor) ELISA Kit

SKU: ELK3212

Size:96T

Reactivity:Human

Alternative Names:TCN3; IF; IFMH; INF; Transcobalamin III; Vitamin B Synthesis

Assay Type:Competitive Inhibition

Sensitivity:0.91 ng/mL

Standard:200 ng/mL

Detection range:3.13-200 ng/mL

Sample type:Serum, plasma and other biological fluids

Assay length:2h

Research Area:Endocrinology;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GIF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GIF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GIF in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P27352