Human Endothelin-2 (EDN2) ELISA Kit

SKU: AE46184HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: P20800

Abbreviation: EDN2

Alternative Names: ET2; PPET2; preproendothelin 2

Application: ELISA

Range: 12.35-1000 pg/mL

Sensitivity: 4.66 pg/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate EDN2 in samples. An antibody specific for EDN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEDN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EDN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EDN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Endothelin 2 is a member of the endothelin protein family of secretory vasoconstrictive peptides. The preproprotein is processed to a short mature form which functions as a ligand for the endothelin receptors that initiate intracellular signaling events. This gene product is involved in a wide range of biological processes, such as hypertension and ovulation. The human endothelins represent a gene family comprised of endothelin-1, endothelin-2, and endothelin-3 . Based on the deduced amino acid sequences of the cloned ET2 and ET3 genes, corresponding proteins have been chemically synthesized and their vasoconstrictor activities studied. Of the 3 isopeptides, ET-2 has the most potent vasoconstrictor activity.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).