Human ECE2(Endothelin Converting Enzyme 2) ELISA Kit

SKU: ELK5083

Size:96T

Reactivity:Human

Alternative Names:Methyltransferase-like region

Assay Type:Competitive Inhibition

Sensitivity:45.4 pg/mL

Standard:10000 pg/mL

Detection range:156.25-10000 pg/mL

Sample type:cell lysates and other biological fluids

Assay length:2h

Research Area:Enzyme & Kinase;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ECE2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ECE2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ECE2 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P0DPD6