Human Dual Specificity Mitogen-activated Protein Kinase 1, MAP2K1 ELISA Kit

SKU: E2488Hu

Size:96 Tests-1 Kit

Background:Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed:29433126). Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

Research_area:Signal Transduction

Applications:ELISA

Target_protein:MAP2K1

Reactivity:Human

Assay type:Sandwich

Sensitivity:11.34ng/L

Detection range:20-7000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Dual specificity mitogen-activated protein kinase 1

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q02750

MASS(Da):43,439

GeneID:5604

Synonyms:CFC3;Dual specificity mitogen-activated protein kinase 1;ERK activator kinase 1;MAP kinase 1;MAP2K1;MAPK/ERK kinase 1;MAPKK 1;MAPKK1;MEK 1;MEK1;MEL;MKK1;PRKMK1;

Gene names:MAP2K1

Standard solution:8000ng/L