Human Dipeptidase 1 (DPEP1) ELISA Kit

SKU: AE46750HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: P16444

Abbreviation: DPEP1

Alternative Names: MBD1; MDP; RDP; OTTHUMP00000175356|dipeptidase 1

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.112 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate DPEP1 in samples. An antibody specific for DPEP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPEP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPEP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPEP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: DPEP1 (EC 3.4.13.11) is a kidney membrane enzyme that hydrolyzes a variety of dipeptides and is implicated in renal metabolism of glutathione and its conjugates, e.g., leukotriene D4 (Kozak and Tate, 1982). DPEP1 is responsible for hydrolysis of the beta-lactam ring of antibiotics, such as penem and carbapenem (Campbell et al., 1984). Earlier, beta-lactamase enzymes were thought to occur only in bacteria, where their probable function was in protecting the organisms against the action of beta-lactam antibiotics. These antibiotics exhibit selective toxicity against bacteria but virtual inertness against many eukaryotic cells (Adachi et al., 1990).

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).