Human Cytochrome P450 11B2, Mitochondrial, ALDOS ELISA Kit

SKU: E2440Hu

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase that catalyzes the biosynthesis of adrenal mineralocorticoid aldosterone (PubMed: 11856349, PubMed: 23322723, PubMed: 1594605, PubMed: 9814506). Catalyzes three sequential oxidative reactions of 11-deoxycorticosterone/21-hydroxyprogesterone, namely 11-beta hydroxylation followed with two successive oxidations at C18 to yield 18-hydroxy and then 18-aldehyde derivatives, resulting in the formation of aldosterone (PubMed: 11856349, PubMed: 23322723, PubMed: 1594605, PubMed: 9814506). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed: 11856349, PubMed: 23322723, PubMed: 1594605, PubMed: 9814506).

Research_area:Others

Applications:ELISA

Target_protein:CYP11B2

Reactivity:Human

Assay type:Sandwich

Sensitivity:10.08ng/L

Detection range:20-6000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Cytochrome P450 11B2, Mitochondrial

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P19099

MASS(Da):57,560

GeneID:1585

Synonyms:ALDOS;Aldosterone Synthase;Aldosterone-synthesizing enzyme;Corticosterone 18-monooxygenase, CYP11B2;CYP11B2;CYPXIB2;Cytochrome P450 11B2, mitochondrial;Cytochrome P-450Aldo;Cytochrome P-450C18;Steroid 11-beta-hydroxylase, CYP11B2;Steroid 18-hydroxylase

Gene names:CYP11B2

Standard solution:6400ng/L