HRP Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L)

SKU: BA1058

Size: 0.5ml

Storage: At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing.

Form: Concentrated, Liquid

Reactivity: Rat

Applications: ELISA,WB

Application Details: Western Blotting: 0.1-0.2μg/ml (ECL detection) Western Blotting: 0.7-3.3μg/ml (DAB detection) ELISA: 0.05-0.5μg/ml (TMB detection)

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Specificity: This HRP conjugated antibody is specific for rat IgG and shows no cross-reactivity with human/bovine/goat/rabbit IgG.

Background: Secondary antibodies offer increased versatility enabling users to use many detection systems such as HRP, AP and fluorescence. They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody, and secondary antibodies' FC regions provide further binding locations for biotin, enable the use of ABC and SABC to further amplify the signal. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They are then purified through immunoaffinity chromatography and modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents.

Immunogen: Rat IgG (whole molecule).

Clonality: Polyclonal

Contents: 0.5 mg of HRP conjugated specific antibody, 0.01M PBS (pH7.4), 50% glycerol.

Purification: The antibody was purified from antisera by immunoaffinity chromatography.

Reconstitution:

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Host: Rabbit

Concentration: 1mg/ml

Conjugate: HRP

Cross Reactivity:

Isotype: IgG

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