HiScript® II Q RT SuperMix for qPCR

R222

Size: 100 rxn (20 μl/rxn)

• Based on high effective HiScript II Reverse Transcriptase.

• High Stability: the recommended reaction temperature is 42℃-55℃.

• Anchored Oligo(dT)23VN: further improves the specificity, efficiency, and success rate of the first-strand cDNA synthesis.

• Ready-to-use SuperMix.

	R211	R212	R222	R223	R232	R233
Application
RT-qPCR			√	√	√	√
universal	√	√
Features
Ready-to use			√	√	√	√
Long cDNA synthesis	√	√			√	√
gDNA wiper		√		√		√
Primers
Oligo (dT)23VN/N6 Mix			√	√
self-selection	√	√			√	√

Table 1. Guidelines for choice of HiScript II two-step RT-PCR/qPCR series.

The Vazyme HiScript II Reverse Transcriptase, optimized from M-MLV (RNase H-) Reverse Transcriptase, is a new generation reverse transcriptase with highly improved heat stability and cDNA synthesis efficiency. The HiScript II Q RT SuperMix for qPCR is specially designed for 2-step RT-qPCR. The 5× Mix contains all necessary components needed for reverse transcription, including Buffer, dNTPs, HiScript II Reverse Transcriptase, RNase inhibitor, and Random primers/Oligo-dT primer mix.

The HiScript II Q RT SuperMix for qPCR has been specially optimized for qPCR. For example, the ratio of Random primers/Oligo-dT primer is optimized to enable cDNA synthesis at any region of the template RNA and to ensure the repeatability of qPCR results. The cDNA products are compatible for SYBR- or probe-based qPCR, such as AceQ qPCR SYBR Green Master Mix (Vazyme, #Q111), ChamQ SYBR qPCR Master Mix (Vazyme, #Q311), ChamQ Color SYBR qPCR Master Mix (Vazyme, #Q411), and AceQ qPCR Probe Master Mix (Vazyme, #Q112).