GIP ELISA kit (Rat)

SKU: CEA882Ra

Size: 96Tests

# of Times Cited in literature: 4

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: GIP

Target Full Name: Gastric Inhibitory Polypeptide

Alternative Names: Incretin hormone; Glucose Dependent Insulinotropic Peptide

Target Species: Rat

Uniprot: Q06145

Gene ID: 25040

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Rat GIP / Gastric Inhibitory Polypeptide

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 61.7-5,000pg/mL

Sensitivity: 26.4pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gastric Inhibitory Polypeptide (GIP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gastric Inhibitory Polypeptide (GIP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Gastric Inhibitory Polypeptide (GIP). No significant cross-reactivity or interference between Gastric Inhibitory Polypeptide (GIP) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gastric Inhibitory Polypeptide (GIP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gastric Inhibitory Polypeptide (GIP) and unlabeled Gastric Inhibitory Polypeptide (GIP) (Standards or samples) with the pre-coated antibody specific to Gastric Inhibitory Polypeptide (GIP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gastric Inhibitory Polypeptide (GIP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gastric Inhibitory Polypeptide (GIP) in the sample.

Research Area: Endocrinology;Gastroenterology;Hormone metabolism;

References Citing This Product: The anti-hyperglycemic efficacy of a lipid-lowering drug Daming capsule and the underlyingsignaling mechanisms in a rat model of diabetes mellitus.

The Effects of Duodenojejunal Omega Switch in Combination with High-Fat Diet and Control Diet on Incretins, Body Weight, and Glucose Tolerance in Sprague-Dawley Rats.

Preserving Duodenal-Jejunal (Foregut) Transit Does Not Impair Glucose Tolerance and Diabetes Remission Following Gastric Bypass in Type 2 Diabetes Sprague …

The Leading Role of Peptide Tyrosine Tyrosine in Glycemic Control After Roux-en-Y Gastric Bypass in Rats