EasyStep Rat VEGFR2(Vascular Endothelial Growth Factor Receptor 2) ELISA Kit

SKU: ELK083ES

Size:96T

Reactivity:Rat

Alternative Names:CD309; FLK1; VEGFR; KDR; A Type III Receptor Tyrosine Kinase; Kinase Insert Domain Receptor; Kinase Insert Domain Receptor; Fetal Liver Kinase-1

Assay Type:Sandwich

Sensitivity:157.5 pg/mL

Standard:50 ng/mL

Detection range:0.78-50 ng/mL

Sample type:Serum, plasma

Assay length:1.5h

Research Area:Signal transduction;CD & Adhesion molecule;Tumor immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VEGFR2, and the Rat VEGFR2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat VEGFR2. After TMB substrate solution is added, only those wells that contain Rat VEGFR2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VEGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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