EasyStep Rat NPT(Neopterin) ELISA Kit

SKU: ELK101ES

Size:96T

Reactivity:Rat

Alternative Names:Neopterin

Assay Type:Competitive Inhibition

Sensitivity:0.4 nmol/L

Standard:30 nmol/L

Detection range:0.469-30 nmol/L

Sample type:Serum, plasma

Assay length:1.5h

Research Area:Cytokine;Infection immunity;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NPT antigen, and the Rat NPT standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat NPT to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat NPT in the samples is then determined by comparing the OD of the samples to the standard curve.

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