CTGF ELISA kit (Human)

SKU: SEA010Hu

Size: 96Tests

# of Times Cited in literature: 24

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: CTGF

Target Full Name: Connective Tissue Growth Factor

Alternative Names: CCN2; HCS24; IGFBP8; NOV2; Insulin-Like Growth Factor-Binding Protein 8; Hypertrophic Chondrocyte-Specific Protein 24; CCN Family Member 2

Target Species: Human

Uniprot: P29279

Gene ID: 1490

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human CTGF / Connective Tissue Growth Factor

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.78-50ng/mL

Sensitivity: 0.32ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Connective Tissue Growth Factor (CTGF). No significant cross-reactivity or interference between Connective Tissue Growth Factor (CTGF) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Connective Tissue Growth Factor (CTGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Connective Tissue Growth Factor (CTGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Connective Tissue Growth Factor (CTGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Connective Tissue Growth Factor (CTGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Cytokine;

References Citing This Product: Promotion of bone formation by naringin in a titanium particle-induced diabetic murine calvarial osteolysis model

Connective tissue growth factor hammerhead ribozyme attenuates human hepatic stellate cell function

Activation of JNK Signaling Mediates Connective Tissue Growth Factor Expression and Scar Formation in Corneal Wound Healing

Clinical significance of inflammatory and fibrogenic cytokines in diabetic nephropathy

Connective tissue growth factor (CTGF/CCN2): A protagonist in cardiac allograft vasculopathy development?

Concentrations of connective tissue growth factor in patients with nonalcoholic fatty liver disease: Association with liver fibrosis

Clinical significance of connective tissue growth factor in hepatitis B virus-induced hepatic fibrosis

Cytokine levels as biomarkers of radiation fibrosis in patients treated with breast radiotherapy

Connective tissue growth factor and cardiac diastolic dysfunction: human data from the Taiwan Diastolic Heart Failure Registry and molecular basis by cellular and animal models

Disruption of collagen homeostasis can reverse established age-related myocardial fibrosis

Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy

Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR

The relationship between anti-vascular endothelial growth factor and fibrosis in proliferative retinopathy: clinical and laboratory evidence

Molecular mechanisms underlying fibrosis and elastin destruction in childhood interstitial lung diseases

Left ventricular reverse remodeling is not related