CR1 ELISA kit (Human)

SKU: SEB123Hu

Size: 96Tests

# of Times Cited in literature: 9

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: CR1

Target Full Name: Complement Receptor 1, Erythrocyte

Alternative Names: CD35; C3BR; KN; C3b/C4b Receptor,Including Knops Blood Group System; Immune Adherence Receptor

Target Species: Human

Uniprot: P17927

Gene ID: 1378

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human CR1 / Complement Receptor 1, Erythrocyte

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.156-10ng/mL

Sensitivity: 0.055ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Receptor 1, Erythrocyte (CR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Receptor 1, Erythrocyte (CR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Complement Receptor 1, Erythrocyte (CR1). No significant cross-reactivity or interference between Complement Receptor 1, Erythrocyte (CR1) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Receptor 1, Erythrocyte (CR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Receptor 1, Erythrocyte (CR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Receptor 1, Erythrocyte (CR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Receptor 1, Erythrocyte (CR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Signal transduction;Tumor immunity;Infection immunity;Immune molecule;Autoimmunity;

References Citing This Product: Ghrelin Alleviates Spinal Cord Injury in Rats Via Its Anti-inflammatory Effects

Complement Receptor 1 Gene Variants Are Associated with Erythrocyte Sedimentation Rate

Cerebrospinal fluid levels of complement proteins C3, C4 and CR1 in Alzheimer's disease

High complement levels in astrocyte‐derived exosomes of Alzheimer disease

Human complement receptor type 1 (CR1) protein levels and genetic variants in chronic Chagas Disease

Complement protein levels in plasma astrocyte-derived exosomes are abnormal in conversion from mild cognitive impairment to Alzheimer's disease …

Traumatic brain injury increases plasma astrocyte‐derived exosome levels of neurotoxic complement proteins

Hepatitis B Virus Infection Among Leprosy Patients: A Case for Polymorphisms Compromising Activation of the Lectin Pathway and Complement Receptors