Cattle ITGb2(Integrin Beta 2) ELISA Kit

SKU: ELK9206

Size:96T

Reactivity:Cattle

Alternative Names:CD18; ITG-B2; LAD; LFA1; MAC1; LCAMB; MF17; LFAb; Complement Component 3 Receptor 3 And 4 Subunit; Lymphocyte Function-Associated Antigen 1; Macrophage Antigen 1

Assay Type:Sandwich

Sensitivity:0.054 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay length:3.5h

Research Area:Signal transduction;CD & Adhesion molecule;Tumor immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle ITGb2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle ITGb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle ITGb2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle ITGb2 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P32592