Cattle IGFBP5(Insulin Like Growth Factor Binding Protein 5) ELISA Kit

SKU: ELK10851

Size:96T

Reactivity:Cattle

Alternative Names:IBP5

Assay Type:Sandwich

Sensitivity:0.52 ng/mL

Standard:100 ng/mL

Detection range:1.57-100 ng/mL

Sample type:Serum, plasma, tissue homogenates and other biological fluids.

Assay length:3.5h

Research Area:Metabolic pathway;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IGFBP5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IGFBP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IGFBP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IGFBP5 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q05717