AP13 ELISA kit (Human)

SKU: CEB887Hu

Size: 96Tests

# of Times Cited in literature: 7

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: AP13

Target Full Name: Apelin 13

Alternative Names: APLN13

Target Species: Human

Uniprot: Q9ULZ1

Gene ID: 8862

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Human AP13 / Apelin 13

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 12.35-1,000pg/mL

Sensitivity: 4.83pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apelin 13 (AP13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apelin 13 (AP13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Apelin 13 (AP13). No significant cross-reactivity or interference between Apelin 13 (AP13) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Apelin 13 (AP13) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Apelin 13 (AP13) and unlabeled Apelin 13 (AP13) (Standards or samples) with the pre-coated antibody specific to Apelin 13 (AP13). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Apelin 13 (AP13) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Apelin 13 (AP13) in the sample.

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Gastroenterology;

References Citing This Product: Apelin in Plasma and Vitreous and in Fibrovascular Retinal Membranes of Patients with Proliferative Diabetic Retinopathy

Effect of Ramadan fasting on serum concentration of apelin-13 and new obesity indices in healthy adult men

The Role of Apelin 13 in Progression of Chronic Kidney Disease.

Role of Circulating Serum Apelin-13 Levels in Glomerulonephritis: A Pilot Study

Biochemical and genetic role of apelin in essential hypertension and acute coronarysyndrome.

Serum concentrations of apelin-17 isoform vary in accordance to blood pressure categories in individuals with obesity class 3

Effect of fermented food consumption on biochemical parameters and adipokines levels

Impact of apelin-13 on the development of coronary artery ectasia