Anti-UMOD Antibody Picoband®

SKU: A01303-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: UMOD

Specificity:

Background: Uromodulin (UMOD), also known as Tamm–Horsfall protein (THP), is a glycoprotein that in humans is encoded by the UMOD gene. The protein encoded by this gene is the most abundant protein in mammalian urine under physiological conditions. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. This protein may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of this protein in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the renal disorders medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN). Alternative splicing of this gene results in multiple transcript variants.

Immunogen: E.coli-derived human UMOD recombinant protein (Position: E43-S618).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Bachmann, S., Mutig, K., Bates, J., Welker, P., Geist, B., Gross, V., Luft, F. C., Alenina, N., Bader, M., Thiele, B. J., Prasadan, K., Raffi, H. S., Kumar, S. Renal effects of Tamm-Horsfall protein (uromodulin) deficiency in mice. Am. J. Physiol. Renal Physiol. 288: F559-F567, 2005. 2. Bernascone, I., Janas, S., Ikehata, M., Trudu, M., Corbelli, A., Schaeffer, C., Rastaldi, M. P., Devuyst, O., Rampoldi, L. A transgenic mouse model for uromodulin-associated kidney diseases shows specific tubulo-interstitial damage, urinary concentrating defect and renal failure. Hum. Molec. Genet. 19: 2998-3010, 2010. 3. Dahan, K., Devuyst, O., Smaers, M., Vertommen, D., Loute, G., Poux, J. M., Viron, B., Jacquot, C., Gagnadoux, M. F., Chauveau, D., Buchler, M., Cochat, P., Cosyns, J. P., Mougenot, B., Rider, M. H., Antignac, C., Verellen-Dumoulin, C., Pirson, Y. A cluster of mutations in the UMOD gene causes familial juvenile hyperuricemic nephropathy with abnormal expression of uromodulin. J. Am. Soc. Nephrol. 14: 2883-2893, 2003.

Uniprot ID: P07911

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 90-110 kDa

Calculated Molecular Weight: 70 kDa

Gene ID: 7369

Protein Name: Uromodulin

Gene Full Name: uromodulin

Synonyms: ADMCKD2; FJHN; HNFJ; MCKD2; THGP; THP; UMOD; uromodulin