Anti-TRMT61B Antibody Picoband®

SKU: A12682-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,IF,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: TRMT61B

Specificity:

Background: Enables mRNA (adenine-N1-)-methyltransferase activity; rRNA (adenine) methyltransferase activity; and tRNA (adenine-N1-)-methyltransferase activity. Involved in mRNA methylation; mitochondrial tRNA methylation; and protein homooligomerization. Located in mitochondrion.

Immunogen: E.coli-derived human TRMT61B recombinant protein (Position: R7-N477).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bar-Yaacov, D., Frumkin, I., Yashiro, I., Chujo, T., Ishigami, Y., Chemla, Y., Blumberg, A., Schlesinger, O., Bieri, P., Greber, B., Ban, N., Zarivach, R., Alfonta, L., Pilpel, Y., Suzuki, T., Mishmar, D. Mitochondrial 16S rRNA is methylated by tRNA methyltransferase TRMT61B in all vertebrates. PLoS Biol. 14: e1002557, 2016. Note: Erratum: PLoS Biol. 15: e1002594, 2017. 2. Chujo, T., Suzuki, T. Trmt61B is a methyltransferase responsible for 1-methyladenosine at position 58 of human mitochondrial tRNAs. RNA 18: 2269-2276, 2012. 3. Gross, M. B. Personal Communication. Baltimore, Md. 6/24/2021.

Uniprot ID: Q9BVS5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 57 kDa

Calculated Molecular Weight:

Gene ID: 55006

Protein Name: tRNA

Gene Full Name: tRNA methyltransferase 61B

Synonyms: TRMT61B