Anti-Transcription factor E2F1 E2F1 Antibody Picoband®

SKU: PA1560

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human , Mouse, Rat

Gene Name: E2F1

Specificity: No cross reactivity with other proteins.

Background: Transcription factor E2F1 is a protein that in humans is encoded by the E2F1 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family of transcription factors appears to play a critical role in the transcription of certain genes required for cell cycle progression. E2F1, the first cloned member of this family, is regulated during the cell cycle at the mRNA level by changes in transcription of the E2F1 gene and at the protein level by complex formation with proteins such as the retinoblastoma gene product (pRB), cyclin A and DP1. E2F1 can override a pRB-induced G1/S block and can behave as an oncogene in certain cells. E2F1 was cloned and was found to contain seven exons. Fluorescence in situ hybridization localized E2F1 to chromosome 20q11. The E2F1 transcription factor can promote proliferation or apoptosis when activated, and is a key downstream target of the retinoblastoma tumour suppressor protein (pRB).

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human E2F1, identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Neuman E, Sellers WR, McNeil JA, Lawrence JB, Kaelin WG Jr (Dec 1996). Structure and partial genomic sequence of the human E2F1 gene. Gene 173 (2): 163–9. 2. Morris, E. J., Ji, J.-Y., Yang, F., DiStefano, L., Herr, A., Moon, N.-S., Kwon, E.-J., Haigis, K. M., Naar, A. M., Dyson, N. J. E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. Nature 455: 552-556, 2008. 3. Neuman, E., Sellers, W. R., McNeil, J. A., Lawrence, J. B., Kaelin, W. G., Jr. Structure and partial genomic sequence of the human E2F1 gene. Gene 173: 163-169, 1996.

Uniprot ID: Q01094

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 60 kDa

Calculated Molecular Weight: 46920 MW

Gene ID: 1869

Protein Name: Transcription factor E2F1

Gene Full Name: E2F transcription factor 1

Synonyms: E2F 1; E2F transcription factor 1; E2F1; PBR3; PRB binding protein E2F 1; RBAP 1; RBAP1; RBBP 3; RBBP3; RBP3; Transcription factor E2F1