Anti-Synaptopodin/SYNPO Antibody Picoband®

SKU: A03154-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: SYNPO

Specificity:

Background: The spine apparatus (SA) is a specialized form of endoplasmic reticulum (ER) that is found in a subpopulation of dendritic spines in central neurons. The SA consists of a series of stacked discs that are though to be connected to each other and to the dendritic system of ER-tubules. The actin binding protein synaptopodin (which has originally been described in podocytes of the kidney) is an essential component of the SA. Mice that lack the gene for synaptopodin do not form a spine apparatus. The SA is believed to play a critical role in learning and memory. In summary, an important function of the spine apparatus is the regulation of plasticity at individual synapses, a process known as metaplasticity. The International Radiation Hybrid Mapping Consortium mapped the SYNPO gene to chromosome 5.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Synaptopodin/SYNPO, which shares 100% and 94.3% amino acid (aa) sequence identity with mouse and rat Synaptopodin/SYNPO, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Cooney; Hurlburt, JL; Selig, DK; Harris, KM; Fiala, JC (2002). Endosomal compartments serve multiple hippocampal dendritic spines from a widespread rather than a local store of recycling membrane. The Journal of neuroscience : the official journal of the Society for Neuroscience 22 (6): 2215–24. 2. Deller; Merten, T; Roth, SU; Mundel, P; Frotscher, M (2000). Actin-associated protein synaptopodin in the rat hippocampal formation: localization in the spine neck and close association with the spine apparatus of principal neurons. The Journal of Comparative Neurology 418 (2): 164–81 3. Gray (1959). Electron microscopy of synaptic contacts on dendrite spines of the cerebral cortex. Nature 183 (4675): 1592–3.

Uniprot ID: Q8N3V7

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 99 kDa

Calculated Molecular Weight: 65411 MW

Gene ID: 11346

Protein Name: Synaptopodin

Gene Full Name: synaptopodin

Synonyms: KIAA1029; synaptopodin; SYNPO