Anti-STK16 Antibody Picoband®

SKU: A10583-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,WB,IHC,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: STK16

Specificity:

Background: Serine/threonine-protein kinase 16 is an enzyme that in humans is encoded by the STK16 gene. Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.Synonyms: MPSK, MPSK1, Myristoylated and palmitoylated serine/threonine-protein kinase, PKL12, Serine/threonine-protein kinase 16, TGF-beta-stimulated factor 1, TSF-1, TSF1

Immunogen: E.coli-derived human STK16 recombinant protein (Position: E109-L268). Human STK16 shares 95.6% and 96.9% amino acid (aa) sequence identity with mouse and rat STK16, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Ligos, J. M., Gerwin, N., Fernandez, P., Gutierrez-Ramos, J. C., Bernad, A. Cloning, expression analysis, and functional characterization of PKL12, a member of a new subfamily of ser/thr kinases. Biochem. Biophys. Res. Commun. 249: 380-384, 1998. 2. Ohta, S., Takeuchi, M., Deguchi, M., Tsuji, T., Gahara, Y., Nagata, K. A novel transcriptional factor with Ser/Thr kinase activity involved in the transforming growth factor (TGF)-beta signalling pathway. Biochem. J. 350: 395-404, 2000.

Uniprot ID: O75716

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 37 kDa

Calculated Molecular Weight: 35 kDa

Gene ID: 8576

Protein Name: Serine/threonine-protein kinase 16

Gene Full Name: serine/threonine kinase 16

Synonyms: hPSK; KRCT; MPSK; MPSK1; PKL12; Protein kinase PKL12; serine/threonine kinase 16; STK16; TGF beta stimulated factor 1; TSF 1; TSF1