Anti-SPART Antibody Picoband®

SKU: A31948-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SPART

Specificity:

Background: Spartin is a protein that in humans is encoded by the SPG20 gene. This gene encodes a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane, and the protein functions in the degradation and intracellular trafficking of EGF receptor. Multiple alternatively spliced variants, encoding the same protein, have been identified. Mutations associated with this gene cause autosomal recessive spastic paraplegia 20 (Troyer syndrome).

Immunogen: E.coli-derived human SPART recombinant protein (Position: H205-V631).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bakowska, J. C., Jenkins, R., Pendleton, J., Blackstone, C. The Troyer syndrome (SPG20) protein spartin interacts with Eps15. Biochem. Biophys. Res. Commun. 334: 1042-1048, 2005. 2. Bakowska, J. C., Wang, H., Xin, B., Sumner, C. J., Blackstone, C. Lack of spartin protein in Troyer syndrome: a loss-of-function disease mechanism? Arch. Neurol. 65: 520-524, 2008. 3. Butler, S., Helbig, K. L., Alcaraz, W., Seaver, L. H., Hsieh, D. T., Rohena, L. Three cases of Troyer syndrome in two families of Filipino descent. Am. J. Med. Genet. 170A: 1780-1785, 2016.

Uniprot ID: Q8N0X7

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 78 kDa

Calculated Molecular Weight: 14377 MW

Gene ID: 23111

Protein Name: Spartin

Gene Full Name: spartin

Synonyms: KIAA0610; SPARTIN; Spartin; SPG20; Spastic paraplegia 20 protein; SPG20; TAHCCP1