Anti-SINHCAF Antibody Picoband®

SKU: A31944-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SINHCAF

Specificity:

Background: FAM60A, also known as Protein FAM60A, or Tera protein homolog, and encoded by the gene FAM60A/C12orf14, is a subunit of the Sin3 deacetylase complex (Sin3/HDAC) that is important for the repression of genes. The SIN3A-HDAC complex deacetylates histones thereby repressing gene transcription. FAM60A specifically impacts genes encoding components of the TGF-beta signaling pathway and promoters of important proteins like cyclin D1. FAM60A activity peaks during G1 and S phases of the cell cycle and is up regulated in many carcinoma and tumor tissues. Additionally, loss of FAM60A leads to a change in cell morphology, an increase in cell migration, increased histone acetylation at the cyclin D1 promoter and elevated levels of cyclin D1 mRNA and protein. Furthermore, depletion of FAM60A altered the periodic association of HDAC1 with the cyclin D1 promoter, increased cyclin D1 expression at all cell cycle phases, and caused premature S phase entry.

Immunogen: E.coli-derived human SINHCAF recombinant protein (Position: M1-W221).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Munoz, I. M., MacArtney, T., Sanchez-Pulido, L., Ponting, C. P., Rocha, S., Rouse, J. Family with sequence similarity 60A (FAM60A) protein is a cell cycle-fluctuating regulator of the SIN3-HDAC1 histone deacetylase complex. J. Biol. Chem. 287: 32346-32353, 2012. 2. Smith, K. T., Sardiu, M. E., Martin-Brown, S. A., Seidel, C., Mushegian, A., Egidy, R., Florens, L., Washburn, M. P., Workman, J. L. Human family with sequence similarity 60 member A (FAM60A) protein: a new subunit of the Sin3 deacetylase complex. Molec. Cell. Proteomics 11: 1815-1828, 2012.

Uniprot ID: Q9NP50

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 28-30 kDa

Calculated Molecular Weight: 14377 MW

Gene ID: 58516

Protein Name: SIN3-HDAC complex-associated factor

Gene Full Name: SIN3-HDAC complex associated factor

Synonyms: SINHCAF; C12orf14; FAM60A; L4; SIN3-HDAC complex-associated factor; Protein FAM60A; Tera protein homolog