Anti-SI Antibody Picoband®

SKU: A04542-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells

Gene Name: SI

Specificity: No cross reactivity with other proteins.

Background: This gene is mapped to 3q26.1. It encodes a sucrase-isomaltase enzyme that is expressed in the intestinal brush border. The encoded protein is synthesized as a precursor protein that is cleaved by pancreatic proteases into two enzymatic subunits sucrase and isomaltase. These two subunits heterodimerize to form the sucrose-isomaltase complex. This complex is essential for the digestion of dietary carbohydrates including starch, sucrose and isomaltose. Mutations in this gene are the cause of congenital sucrase-isomaltase deficiency.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SI, which shares 86.1% amino acid (aa) sequence identity with rat SI.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Birkenmeier, E. H., Rowe, L. B., Leeper, L. L., Henning, S. J. The sucrase-isomaltase structural gene (Si-s) and a regulatory gene (Si-r) are closely linked to esterase-26 (Es-26) on mouse chromosome 3. Mammalian Genome 4: 531-536, 1993. 2. Chantret, I., Lacasa, M., Chevalier, G., Ruf, J., Islam, I., Mantei, N., Edwards, Y., Swallow, D., Rousset, M. Sequence of the complete cDNA and the 5-prime structure of the human sucrase-isomaltase gene: possible homology with a yeast glucoamylase. Biochem. J. 285: 915-923, 1992. 3. Davis, M. B., Green, F. R., West, L. F., Kearney, A., Povey, S., Swallow, D. M. Mapping of the gene encoding human sucrase-isomaltase (SI) to chromosome 3q25-26. (Abstract) Cytogenet. Cell Genet. 46: 604, 1987.

Uniprot ID: P14410

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 209 kDa

Calculated Molecular Weight: 209 kDa

Gene ID: 6476

Protein Name: Sucrase-isomaltase, intestinal [Cleaved into: Sucrase

Gene Full Name: sucrase-isomaltase

Synonyms: sucrase-isomaltase; SI