Anti-SH2D4A Antibody Picoband®

SKU: A09094-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SH2D4A

Specificity:

Background: SH2 domain-containing protein 4A is a protein that in humans is encoded by the SH2D4A gene. Using exon trapping and exon linking at chromosome 8p22 cloned SH2D4A, which they called SH2A. The deduced 454-amino acid protein contains a single SH2 domain. RT-PCR and Northern blot analysis detected ubiquitous expression of 3 SH2A transcripts, as well as aberrant expression in some cancer cell lines. Dai et al. (2002) concluded that SH2A is a docking protein that may be involved in signal transduction.

Immunogen: E.coli-derived human SH2D4A recombinant protein (Position: R42-E454).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Dai, S., Zhao, Y., Ding, Q. A novel member of SH2A signaling protein family: cloning and characterization of SH2 gene. Chinese J. Med. Genet. 19: 458-462, 2002. 2. Lapinski, P. E., Oliver, J. A., Kamen, L. A., Hughes, E. D., Saunders, T. L., King, P. D. Genetic analysis of SH2D4A, a novel adapter protein related to T cell-specific adapter and adapter protein in lymphocytes of unknown function, reveals a redundant function in T cells. J. Immun. 181: 2019-2027, 2008. 3. Li, T., Li, W., Lu, J., Liu, H., Li, Y., Zhao, Y. SH2D4A regulates cell proliferation via the ER-alpha/PLC-gamma/PKC pathway. BMB Rep. 42: 516-522, 2009.

Uniprot ID: Q9H788

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 50 kDa

Calculated Molecular Weight:

Gene ID: 63898

Protein Name: SH2 domain-containing protein 4A

Gene Full Name: SH2 domain containing 4A

Synonyms: SH2D4A; PPP1R38; SH2A; Protein SH(2)A