Anti-SF3A2 Antibody Picoband®

SKU: A11289-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,IP,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SF3A2

Specificity:

Background: Splicing factor 3A subunit 2 is a protein that in humans is encoded by the SF3A2 gene. This gene encodes subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein.

Immunogen: E.coli-derived human SF3A2 recombinant protein (Position: R29-P256).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bennett, M., Reed, R. Correspondence between a mammalian spliceosome component and an essential yeast splicing factor. Science 262: 105-108, 1993. 2. Dresser, D. W., Hacker, A., Lovell-Badge, R., Guerrier, D. The genes for a spliceosome protein (SAP62) and the anti-mullerian hormone (AMH) are contiguous. Hum. Molec. Genet. 4: 1613-1618, 1995. 3. Dresser, D. W., Jamin, S. P., Atkins, C. J., Guerrier, D. An expressed GNRP-like gene shares a bi-ional promoter with SF3A2 (SAP62) immediately upstream of AMH. Gene 277: 163-173, 2001.

Uniprot ID: Q15428

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 66 kDa

Calculated Molecular Weight:

Gene ID: 8175

Protein Name: Splicing factor 3A subunit 2

Gene Full Name: splicing factor 3a subunit 2

Synonyms: PRP11; PRPF11; SAP 62; SAP62; SF3A2; SF3a66; Splicing factor 3A subunit 2