Anti-SCOP/PHLPP1 Picoband® Antibody

SKU: A02430-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: PHLPP1

Specificity: No cross reactivity with other proteins.

Background: The PHLPP isoforms (PH domain and Leucine rich repeat Protein Phosphatases) are a pair of protein phosphatases, PHLPP1 and PHLPP2, that are important regulators of Akt serine-threonine kinases (Akt1, Akt2, Akt3) and conventional/novel protein kinase C (PKC) isoforms. It is mapped to 18q21.33. This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.

Immunogen: E.coli-derived human SCOP/PHLPP1 recombinant protein (Position: R1083-A1436).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Brognard, J., Sierecki, E., Gao, T., Newton, A. C. PHLPP and a second isoform, PHLPp2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms. Molec. Cell 25: 917-931, 2007. 2. Chen, H. H., Handel, N., Ngeow, J., Muller, J., Huhn, M., Yang, H.-T., Heindl, M., Berbers, R.-M., Hegazy, A. N., Kionke, J., Yehia, L., Sack, U., and 15 others. Immune dysregulation in patients with PTEN hamartoma tumor syndrome.: analysis of FOXP3 regulatory T cells. J. Allergy Clin. Immun. 139: 607-620, 2017. 3. Cheng, H.-Y. M., Papp, J. W., Varlamova, O., Dziema, H., Russell, B., Curfman, J. P., Nakazawa, T., Shimizu, K., Okamura, H., Impey, S., Obrietan, K. microRNA modulation of circadian-clock period and entrainment. Neuron 54: 813-829, 2007.

Uniprot ID: O60346

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 140 kDa

Calculated Molecular Weight: 38779 MW

Gene ID: 23239

Protein Name: PH domain leucine-rich repeat-containing protein phosphatase 1

Gene Full Name: PH domain and leucine rich repeat protein phosphatase 1

Synonyms: hSCOP; KIAA0606; PHLPP; PHLPP1; PLEKHE1; SCOP