Anti-RUNX1/AML1 Antibody Picoband®

SKU: PB9157

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: IHC,IHC-F,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat

Gene Name: RUNX1

Specificity: No cross reactivity with other proteins.

Background: Runt-related transcription factor 1 (RUNX1), also known as AML1 or CBFA2, is a protein that in humans is encoded by the RUNX1 gene. It belongs to the Runt-related transcription factor (RUNX) family of genes which are also called core binding factor-α (CBFα). RUNX1 is mapped to 21q22.12. RUNX1 is a transcription factor that regulates the differentiation of hematopoietic stem cells into mature blood cells. RUNX proteins form a heterodimeric complex with CBFβ which confers increased DNA binding and stability to the complex. Chromosomal translocations involving the RUNX1 gene are associated with several types of leukemia including M2 AML. Mutations in RUNX1 are implicated in cases of breast cancer.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human RUNX1, identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Avramopoulos D, Cox T, Blaschak JE, Chakravarti A, Antonarakis SE (October 1992). Linkage mapping of the AML1 gene on human chromosome 21 using a DNA polymorphism in the 3' untranslated region. Genomics 14 (2): 506–7. 2. Okuda T, Nishimura M, Nakao M, Fujita Y (October 2001). RUNX1/AML1: a central player in hematopoiesis. Int. J. Hematol. 74 (3): 252–7. 3. The Cancer Genome Atlas Network (2012). Comprehensive molecular portraits of human breast tumours. Nature (Nature Publishing Group) 490 (7418): 61–70.

Uniprot ID: Q01196

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 55 kDa

Calculated Molecular Weight: 48737 MW

Gene ID: 861

Protein Name: Runt-related transcription factor 1

Gene Full Name: RUNX family transcription factor 1

Synonyms: AML1; AML1 EVI 1; AMLCR1; CBF alpha 2; CBFA2; EVI 1; Oncogene AML 1; PEA2 alpha B; PEBP2 alpha B; PEBP2aB; RUNX1; RUNX1 (middle)