Anti-ROBO4 Antibody Picoband®

SKU: A04479-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ROBO4

Specificity:

Background: Roundabout homolog 4 is a protein that in humans is encoded by the ROBO4 gene. Predicted to enable cell-cell adhesion mediator activity. Involved in angiogenesis and establishment of endothelial barrier. Located in extracellular exosome. Implicated in aortic valve disease 3.

Immunogen: E.coli-derived human ROBO4 recombinant protein (Position: D108-A933).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bedell, V. M., Yeo, S.-Y., Park, K. W., Chung, J., Seth, P., Shivalingappa, V., Zhao, J., Obara, T., Sukhatme, V. P., Drummond, I. A., Li, D. Y., Ramchandran, R. roundabout4 is essential for angiogenesis in vivo. Proc. Nat. Acad. Sci. 102: 6373-6378, 2005. 2. Gould, R. A., Aziz, H., Woods, C. E., Seman-Senderos, M. A., Sparks, E., Preuss, C., Wunnemann, F., Bedja, D., Moats, C. R., McClymont, S. A., Rose, R., Sobreira, N., and 33 others. ROBO4 variants predispose individuals to bicuspid aortic valve and thoracic aortic aneurysm. Nature Genet. 51: 42-50, 2019. 3. Huminiecki, L., Gorn, M., Suchting, S., Poulsom, R., Bicknell, R. Magic roundabout is a new member of the roundabout receptor family that is endothelial specific and expressed at sites of active angiogenesis. Genomics 79: 547-552, 2002.

Uniprot ID: Q8WZ75

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 107 kDa

Calculated Molecular Weight: 128770 MW

Gene ID: 54538

Protein Name: Roundabout homolog 4

Gene Full Name: roundabout guidance receptor 4

Synonyms: ROBO4; UNQ421/PRO3674; Roundabout homolog 4; Magic roundabout