Anti-RNF25 Antibody Picoband®

SKU: A10595-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: RNF25

Specificity:

Background: E3 ubiquitin-protein ligase RNF25 is an enzyme that in humans is encoded by the RNF25 gene. The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination.

Immunogen: E.coli-derived human RNF25 recombinant protein (Position: E44-R344). Human RNF25 shares 83.7% amino acid (aa) sequence identity with mouse RNF25.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Asamitsu, K., Tetsuka, T., Kanazawa, S., Okamoto, T. RING finger protein AO7 supports NF-kappa-B-mediated transcription by interacting with the transactivation domain of the p65 subunit. J. Biol. Chem. 278: 26879-26887, 2003. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 9/18/2014. 3. Lorick, K. L., Jensen, J. P., Fang, S., Ong, A. M., Hatakeyama, S., Weissman, A. M. RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. Proc. Nat. Acad. Sci. 96: 11364-11369, 1999.

Uniprot ID: Q96BH1

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 62 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 64320

Protein Name: E3 ubiquitin-protein ligase RNF25

Gene Full Name: ring finger protein 25

Synonyms: RNF25; E3 ubiquitin-protein ligase RNF25; RING finger protein 25; RING-type E3 ubiquitin transferase RNF25