Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband® (monoclonal, 4F3)

SKU: M04147

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: RNH1

Specificity: No cross reactivity with other proteins.

Background: Ribonuclease inhibitor is an enzyme that in humans is encoded by the RNH1 gene. Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular Rnases. In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin. Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human RNH1, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: RNH1 ribonuclease/angiogenin inhibitor 1. 2. Weremowicz S, Fox EA, Morton CC, Vallee BL (1991). The placental ribonuclease inhibitor (RNH) gene is located on chromosome subband 11p15.5.. Genomics 8(4): 717–21. 3. Zneimer SM, Crawford D, Schneider NR, Beutler B (1991). Mapping of the human ribonuclease inhibitor gene (RNH) to chromosome 11p15 by in situ hybridization.. Genomics 8 (1): 175–8.

Uniprot ID: P13489

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 4F3

Observed Molecular Weight: 45 kDa

Calculated Molecular Weight:

Gene ID: 6050

Protein Name: Ribonuclease inhibitor

Gene Full Name: ribonuclease/angiogenin inhibitor 1

Synonyms: Placental RNase inhibitor; PRI; RAI; Ribonuclease inhibitor; RNH; RNH1