Anti-RASAL1/RASAL1 Antibody Picoband®

SKU: A06423-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml

Gene Name: RASAL1

Specificity: No cross reactivity with other proteins.

Background: The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. And it is mapped to 12q24.13. These proteins stimulate the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This particular family member contains domains which are characteristic of the GAP1 subfamily of RasGAP proteins but, in contrast to the other GAP1 family members, this protein is strongly and selectively expressed in endocrine tissues. Alternatively spliced transcript variants that encode different isoforms have been described.

Immunogen: E. coli-derived human RASAL1 recombinant protein (Position: D721-A798).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Allen, M., Chu, S., Brill, S., Stotler, C., Buckler, A. Restricted tissue expression pattern of a novel human rasGAP-related gene and its murine ortholog. Gene 218: 17-25, 1998. 2. Bechtel, W., McGoohan, S., Zeisberg, E. M., Muller, G. A., Kalbacher, H., Salant, D. J., Muller, C. A., Kalluri, R., Zeisberg, M. Methylation determines fibroblast activation and fibrogenesis in the kidney. Nature Med. 16: 544-550, 2010. 3. Jin, H., Wang, X., Ying, J., Wong, A. H. Y., Cui, Y., Srivastava, G., Shen, Z.-Y., Li, E.-M., Zhang, Q., Jin, J., Kupzig, S., Chan, A. T. C., Cullen, P. J., Tao, Q. Epigenetic silencing of a Ca(2+)-regulated Ras GTPase-activating protein RASAL defines a new mechanism of Ras activation in human cancers. Proc. Nat. Acad. Sci. 104: 12353-12358, 2007.

Uniprot ID: O95294

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 90 kDa

Calculated Molecular Weight:

Gene ID: 8437

Protein Name: RasGAP-activating-like protein 1

Gene Full Name: RAS protein activator like 1

Synonyms: RAS protein activator like 1; RASAL; RASAL1