Anti-PRPF4 Antibody Picoband®

SKU: A08225-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml,Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PRPF4

Specificity:

Background: U4/U6 small nuclear ribonucleoprotein Prp4 is a protein that in humans is encoded by the PRPF4 gene. he protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human PRPF4 recombinant protein (Position: R5-H461).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chen, X., Liu, Y., Sheng, X., Tam, P. O. S., Zhao, K., Chen, X., Rong, W., Liu, Y., Liu, X., Pan, X., Chen, L. J., Zhao, Q., Vollrath, D., Pang, C. P., Zhao, C. PRPF4 mutations cause autosomal dominant retinitis pigmentosa. Hum. Molec. Genet. 23: 2926-2939, 2014. 2. Gonzalez-Santos, J. M., Wang, A., Jones, J., Ushida, C., Liu, J., Hu, J. Central region of the human splicing factor Hprp3p interacts with Hprp4p. J. Biol. Chem. 277: 23764-23772, 2002. 3. Heng, H. H. Q., Wang, A., Hu, J. Mapping of the human HPRP3 and HPRP4 genes encoding U4/U6-associated splicing factors to chromosomes 1q21.1 and 9q31-q33. Genomics 48: 273-275, 1998.

Uniprot ID: O43172

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 55 kDa

Calculated Molecular Weight:

Gene ID: 9128

Protein Name: U4/U6 small nuclear ribonucleoprotein Prp4

Gene Full Name: pre-mRNA splicing tri-snRNP complex factor PRPF4

Synonyms: HPRP4; HPRP4P; PRP4; PRP4 homolog; Prp4p; PRPF4; U4/U6 snRNP 60 kDa protein; WD splicing factor Prp4