Anti-PP11/ENDOU Antibody Picoband®

SKU: A07350-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ENDOU

Specificity:

Background: Endonuclease, poly(U) specific is a protein that in humans is encoded by the ENDOU gene. This gene encodes a protein with endoribonuclease activity that binds polyuridine-enriched single-stranded RNA. This gene was initially characterized based on its high expression in placenta but was mischaracterized as a serine protease. In mouse, this gene promotes tolerance to self-antigens by regulating B cell activation-induced cell death (AICD). The protein may be useful as a tumor marker. Multiple alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene.

Immunogen: E.coli-derived human PP11/ENDOU recombinant protein (Position: K20-T386).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gross, M. B. Personal Communication. Baltimore, Md. 2/24/2014. 2. Grundmann, U., Romisch, J., Siebold, B., Bohn, H., Amann, E. Cloning and expression of a cDNA encoding human placental protein 11, a putative serine protease with diagnostic significance as a tumor marker. DNA Cell Biol. 9: 243-250, 1990. 3. Laneve, P., Gioia, U., Ragno, R., Altieri, F., Di Franco, C., Santini, T., Arceci, M., Bozzoni, I., Caffarelli, E. The tumor marker human placental protein 11 is an endoribonuclease. J. Biol. Chem. 283: 34712-34719, 2008.

Uniprot ID: P21128

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 47 kDa

Calculated Molecular Weight: 108320 MW

Gene ID: 8909

Protein Name: Uridylate-specific endoribonuclease

Gene Full Name: endonuclease, poly(U) specific

Synonyms: 26 serine protease; ENDOU; P11; Placental protein 11; PP11; Protein endoU; PRSS26