Anti-PLS3 Antibody Picoband®

SKU: A04134-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IP,IF,ICC,WB

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 2-4 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PLS3

Specificity:

Background: Plastin-3 is a highly conserved protein that in humans is encoded by the PLS3 gene on the X chromosome. Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.

Immunogen: E.coli-derived human PLS3 recombinant protein (Position: M1-D580). Human PLS3 shares 99% and 97.9% amino acid (aa) sequence identity with mouse and rat PLS3, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Ackermann, B., Krober, S., Torres-Benito, L., Borgmann, A., Peters, M., Barkooie, S. M. H., Tejero, R., Jakubik, M., Schreml, J., Milbradt, J., Wunderlich, T. F., Riessland, M., Tabares, L., Wirth, B. Plastin 3 ameliorates spinal muscular atrophy via delayed axon pruning and improves neuromuscular junction functionality. Hum. Molec. Genet. 22: 1328-1347, 2013. 2. Lin, C.-S., Aebersold, R. H., Kent, S. B., Varma, M., Leavitt, J. Molecular cloning and characterization of plastin, a human leukocyte protein expressed in transformed human fibroblasts. Molec. Cell. Biol. 8: 4659-4668, 1988. 3. Lin, C.-S., Aebersold, R. H., Leavitt, J. Correction of the N-terminal sequences of the human plastin isoforms by using anchored polymerase chain reaction: identification of a potential calcium-binding domain. Molec. Cell. Biol. 10: 1818-1821, 1990.

Uniprot ID: P13797

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 71 kDa

Calculated Molecular Weight: 71,69,66 kDa

Gene ID: 5358

Protein Name: Plastin-3

Gene Full Name: plastin 3

Synonyms: PLS3; Plastin-3; T-plastin