Anti-PLK1 Antibody Picoband® (monoclonal, 6D11)

SKU: M00182

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Monkey

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml

Gene Name: PLK1

Specificity: No cross reactivity with other proteins.

Background: Serine/threonine-protein kinase PLK1, also known as polo-like kinase 1 (PLK-1) or serine/threonine-protein kinase 13 (STPK13), is an enzyme that in humans is encoded by the PLK1 (polo-like kinase 1) gene. Plk1 is an early trigger for G2/M transition. It supports the functional maturation of the centrosome in late G2/early prophaseand establishment of the bipolar spindle. Also, Plk1 phosphorylates and activates cdc25C, a phosphatase that dephosphorylates and activates the cyclinB/cdc2 complex. Studies have shown that the loss of PLK1 expression can inducepro-apoptotic pathways and inhibit growth. Based on yeast and murine studies of meiosis, human PLK1 may also have a regulatory function in meiosis.

Immunogen: E.coli-derived human PLK1 recombinant protein (Position: K86-N430). Human PLK1 shares 95.4% and 96.2% amino acid (aa) sequence identity with mouse and rat PLK1, respectively.

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Reference: 1. Holtrich U, Wolf G, Bräuninger A, Karn T, Böhme B, Rübsamen-Waigmann H et al. (March 1994). Induction and down-regulation of PLK, a human serine/threonine kinase expressed in proliferating cells and tumors. Proc. Natl. Acad. Sci. U.S.A. 91(5): 1736–40. 2. Soung NK, Park JE, Yu LR, Lee KH, Lee JM, Bang JK et al. (April 2009). Plk1-dependent and -independent roles of an ODF2 splice variant, hCenexin1, at the centrosome of somatic cells. Dev. Cell 16(4): 539–50.

Uniprot ID: P53350

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 6D11

Observed Molecular Weight: 68 kDa

Calculated Molecular Weight: 46659 MW

Gene ID: 5347

Protein Name: Serine/threonine-protein kinase PLK1

Gene Full Name: polo like kinase 1

Synonyms: PLK; PLK 1; PLK1; Polo like kinase 1; STPK13