Anti-PAX3 Antibody Picoband®

SKU: A00285-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PAX3

Specificity:

Background: The PAX3 (paired box gene 3) gene encodes a member of the paired box or PAX family of transcription factors. This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.

Immunogen: E.coli-derived human PAX3 recombinant protein (Position: Q306-E420). Human PAX3 shares 99.1% amino acid (aa) sequence identity with mouse PAX3.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Asher, J. H., Jr., Harrison, R. W., Morell, R., Carey, M. L., Friedman, T. B. Effects of Pax3 modifier genes on craniofacial morphology, pigmentation, and viability: a murine model of Waardenburg syndrome variation. Genomics 34: 285-298, 1996. 2. Asher, J. H., Jr., Sommer, A., Morrell, R., Friedman, T. B. Missense mutation in the paired domain of PAX3 causes craniofacial-deafness-hand syndrome. Hum. Mutat. 7: 30-35, 1996. 3. Baldwin, C. T., Hoth, C. F., Amos, J. A., da-Silva, E. O., Milunsky, A. An exonic mutation in the HuP2 paired domain gene causes Waardenburg's syndrome. Nature 355: 637-638, 1992.

Uniprot ID: P23760

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 69 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 5077

Protein Name: Paired box protein Pax-3

Gene Full Name: paired box 3

Synonyms: CDHS; HUP2; paired box 3; Paired box protein Pax 3; PAX3; WS1