Anti-PARP16 Antibody

SKU: A10545-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: IHC,Flow Cytometry,ELISA

Application Details: Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: PARP16

Specificity:

Background: Enables kinase binding activity; pentosyltransferase activity; and protein serine/threonine kinase activator activity. Involved in IRE1-mediated unfolded protein response; negative regulation of cytoplasmic translation; and protein auto-ADP-ribosylation. Located in endoplasmic reticulum tubular network and nuclear envelope.

Immunogen: E.coli-derived human PARP16 recombinant protein (Position: A9-Y254). Human PARP16 shares 92.3% and 90.2% amino acid (aa) sequence identity with mouse and rat PARP16, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Di Paola, S., Micaroni, M., Di Tullio, G., Buccione, R., Di Girolamo, M. PARP16/ARTD15 is a novel endoplasmic-reticulum-associated mono-ADP-ribosyltransferase that interacts with, and modifies karyopherin-beta-1. PLoS One 7: e37352, 2012. 2. Gross, M. B. Personal Communication. Baltimore, Md. 4/20/2023. 3. Jwa, M., Chang, P. PARP16 is a tail-anchored endoplasmic reticulum protein required for the PERK- and IRE1-alpha-mediated unfolded protein response. Nature Cell Biol. 14: 1223-1230, 2012. Note: Erratum: Nature Cell Biol. 15: 123 only, 2013.

Uniprot ID: Q8N5Y8

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight:

Calculated Molecular Weight:

Gene ID: 54956

Protein Name: Protein mono-ADP-ribosyltransferase PARP16

Gene Full Name: poly(ADP-ribose) polymerase family member 16

Synonyms: PARP16; ARTD15; C15orf30; Protein mono-ADP-ribosyltransferase PARP16; EC 2.4.2.-; ADP-ribosyltransferase diphtheria toxin-like 15; Poly [ADP-ribose] polymerase 16; PARP-16