Anti-PAAF1 Antibody Picoband®

SKU: A12843-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PAAF1

Specificity:

Background: Proteasomal ATPase-associated factor 1 is an enzyme that in humans is encoded by the PAAF1 gene. This gene encodes a WD repeat-containing protein involved in regulation of association of proteasome components. During HIV infection, the encoded protein is thought to promote provirus transcription through recruitment of the 19S regulatory complex. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Immunogen: E.coli-derived human PAAF1 recombinant protein (Position: H27-L392).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gross, M. B. Personal Communication. Baltimore, Md. 2/28/2022. 2. Lassot, I., Latreille, D., Rousset, E., Sourisseau, M., Linares, L. K., Chable-Bessia, C., Coux, O., Benkirane, M., Kiernan, R. E. The proteasome regulates HIV-1 transcription by both proteolytic and nonproteolytic mechanisms. Molec. Cell 25: 369-383, 2007. 3. Nakamura, M., Basavarajaiah, P., Rousset, E., Beraud, C., Latreille, D., Henaoui, I.-S., Lassot, I., Mari, B., Kiernan, R. Spt6 levels are modulated by PAAF1 and proteasome to regulate the HIV-1 LTR. Retrovirology 9: 13, 2012.

Uniprot ID: Q9BRP4

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 42 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 80227

Protein Name: Proteasomal ATPase-associated factor 1

Gene Full Name: proteasomal ATPase associated factor 1

Synonyms: PAAF1; WDR71; Proteasomal ATPase-associated factor 1; Protein G-16; WD repeat-containing protein 71