Anti-P4HB Antibody Picoband®

SKU: A02335-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Monkey,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.25μg/ml, Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: P4HB

Specificity:

Background: Protein disulfide-isomerase, also known as the beta-subunit of prolyl 4-hydroxylase (P4HB), is an enzyme that in humans encoded by the P4HB gene. This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Immunogen: E.coli-derived human P4HB recombinant protein (Position: A146-D363).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Cheng, S. Y., Gong, Q. H., Parkinson, C., Robinson, E. A., Appella, E., Merlino, G. T., Pastan, I. The nucleotide sequence of a human cellular thyroid hormone-binding protein present in endoplasmic reticulum. J. Biol. Chem. 262: 11221-11227, 1987. 2. Koivu, J., Myllyla, R., Halaakoski, T., Pihlajaniemi, T., Tasanen, K., Kivirikko, K. I. A single polypeptide acts both as the beta subunit of prolyl 4-hydroxylase and as a protein disulfide-isomerase. J. Biol. Chem. 262: 6447-6449, 1987. 3. Mezghrani, A., Fassio, A., Benham, A., Simmen, T., Braakman, I., Sitia, R. Manipulation of oxidative protein folding and PDI redox state in mammalian cells. EMBO J. 20: 6288-6296, 2001.

Uniprot ID: P07237

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 57 kDa

Calculated Molecular Weight:

Gene ID: 5034

Protein Name: Protein disulfide-isomerase

Gene Full Name: prolyl 4-hydroxylase subunit beta

Synonyms: Cell and organelle markers; DSI; endoplasmic reticulum marker; ERBA2L; GIT; P4HB; P4Hbeta; p55; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB; Protein disulfide isomerase